Adventitious viruses detection by PCR 
As recommended in “Points to Consider on the Characterisation of Cell Lines Used to Produce Biologicals”, Texcell provides a wide range of quantitative real-time polymerase chain reaction (Q-PCR) tests for the detection of human viruses. We use TaqMan® PCR to count the number of copies of the virus sequence.
The PCR enables rapid detection of specific viruses in test products. Due to the assay's sensitivity, Texcell’s procedures take great care to avoid any cross-contamination of the end product. Reagents, samples and PCR products are prepared and handled in three separate laboratories and a comprehensive set of negative controls is included to ensure that contamination has been avoided or detected. For RNA viruses, after genome extraction from the test article, the RNA is incubated with the reverse transcriptase to form a complementary DNA (cDNA), which is further amplified by Q-PCR (RTQ-PCR).
Human |
Bovine viruses |
Porcine viruses |
Murine viruses |
Simian viruses |
HIV1 HIV2 HTLV-1 & 2 |
BPyV BVDV |
PPV PRV |
MVM MLV |
Simian Herpes Virus SV40 SCMV |
HAV HBV HCV EBV CMV HHV6 HHV7 HHV8 B19 AAV Adeno5 Poliomavirus Papillomavirus Enterovirus |
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SIV1, SIV2 SRV1-2-3 Spumavirus SMRV |
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